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Method Development for Determination of Dexamethasone and Prednisolone in Traditional Drug by High-Performance Liquid Chromatography
  • 4.12.2017
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Method Development for Determination of Dexamethasone and Prednisolone in Traditional Drug by High-Performance Liquid Chromatography
Owner / Producer Owner / Producer
Jiranuch Jamtaweekul, Sumate Thiangthum, Waliluk Matapatara, Chitra Chaiyawat
Year Year
2015
Pages Pages
17
Details

The quantitative determination of the adulterated dexamethasone and prednisolone in traditional drug was developed by using high performance liquid chromatography (HPLC) with reversed phase C8 column (Hypersil BDS) and a mixture of acetonitrile and purified water as a mobile phase. The sample solution was cleaned up by solid phase extraction (SPE). The method validation data showed good linearity at the concentration range of 250-12,500 μg/L with the correlation coefficient of 0.9987 and 0.9988 for dexamethasone and prednisolone, respectively. Accuracy and precision were performed by adding 500, 6,000 and 12,000 μg/L of each of dexamethasone and prednisolone to sample matrix. The percentages of recovery were 100.30-104.53 and 100.02-102.18 for dexamethasone and prednisolone, respectively. The relative standard deviations of dexamethasone and prednisolone were 0.04-2.01 and 0.15-2.47, respectively. The limit of quantitation of dexamethasone and prednisolone are 250.14 and 249.30 μg/L, respectively. Therefore, the developed HPLC method is suitable for surveillance of the dexamethasone and prednisolone adulterants in traditional herbal medicines.

https://he02.tci-thaijo.org/index.php/dmsc/article/view/242649